Publications - Nano-Biomolecular Precision Lab

[For a full list of publications on Google Scholar, click here]

	Nguyen LT, Macaluso NC, Rakestraw NR, Carman DR, Pizzano BLM, Hautamaki RC, and Jain PK* Harnessing Non-canonical crRNAs to Improve Functionality of Cas12a Orthologs”, Cell Reports, 2024, 43 (2), doi.org/10.1016/j.celrep.2024.113777. [View] [SSRN-2023][medRxiv-2021] [*Corresponding author]
	Rananaware SR, Vesco EK, Shoemaker GM, Anekar SS, Sandoval LSW, Meister KS, Macaluso NC, Nguyen LT, and Jain, PK, Programmable RNA detection with CRISPR-Cas12a. Nature Communications, 2023, 14 (5409), doi.org/10.1038/s41467-023-41006-1. [View] [Research Square, 2023] [bioRxiv,* 2023] *[Corresponding author]**
	Shashank PR, Parker BM^§, Rananaware SR^§, Plotkin D, Couch C, Yang LG, Nguyen LT, Prasannakumar NR, Braswell WE, Jain PK^‡, Kawahara AY, CRISPR-based diagnostics detects invasive insect pests Molecular Ecology Resources,* 2023, 00, 1–15, doi.org/10.1111/1755-0998.13881. [View] [bioRxiv, 2023][^§Equal contributions][^‡Senior author]
	Nguyen LT^§, Rananaware SR^§, Yang LG^§, Macaluso NC^§, Ocana-Ortiz JE, Meister KS, Pizzano BLM, Sandoval LSW, Hautamaki RC, Fang ZR, Joseph SM, Shoemaker GM, Carman DR, Chang L, Rakestraw NR, Zachary JF, Guerra S, Perez A, Jain PK, Engineering Highly Thermostable Cas12b via De Novo Structural Analyses for One-Pot Detection of Nucleic Acids Cell Reports Medicine, 2023, 4(5), 101037 https://doi.org/10.1016/j.xcrm.2023.101037 [View] [Highlighted on the cover] [medRxiv, 2022] [SSRN, 2023] [Corresponding author][^§Equal contributions]
	Shen Y, Khatri B, Rananaware S, Li D, Ostrov DA, Jain PK^‡, Lessard CJ, and Nguyen CQ* Ancestral origins are associated with SARS-CoV-2 susceptibility and protection in a Florida patient population PLOS One, 2023, https://doi.org/10.1371/journal.pone.0276700 [^‡Senior author] [Download pdf] [View] [bioRxiv version-2022]
	Wei X, Wang X, Zhang Z, Luo Y, Wang Z, Xiong W, Jain PK^‡, Monnier JR, Wang H, Hu TY, Tang C, Albrecht H, and Liu C* A Click Chemistry Amplified Nanopore (CAN) Assay for Ultrasensitive Quantification of HIV p24 Antigen in Clinical Samples Nature Communications, 2022, 13, 6852 https://doi.org/10.1038/s41467-022-34273-x [^‡Senior author]
	Nguyen, LT, Macaluso N, Pizzano B, Cash M, Spacek J, Karasek J, Miller MR, Lednicky JA, Dinglasan RD, Salemi M, and Jain PK* A Thermostable Cas12b from Brevibacillus Leverages One-pot Discrimination of SARS-CoV-2 Variants of Concern eBioMedicine- THE LANCET, 2022, https://doi.org/10.1016/j.ebiom.2022.103926 [*Corresponding author] [*medRxiv] [Preprints with THE LANCET]* [Download pdf] [View]
	Nguyen, LT, Rananaware SR, Pizzano BLM, Brandon ST, and Jain PK* Clinical validation of engineered CRISPR/Cas12a for rapid SARS-CoV-2 detection. Communications Medicine- Nature, 2022, https://doi.org/10.1038/s43856-021-00066-4 [*Corresponding author] [Download pdf] [View]
	Chiu CW, Xian M, Stephany JL, Xia X, Chiang CC, Ren F, Tsai CT, Shan SS, Liao YT, Esquivel-Upshaw JF, Rananaware SR, Nguyen LT, Macaluso NC, Jain PK^‡, Cash MN, Mavian CN, Salemi M, Leon ME, Chang CW, Lin J, and Pearton SJ* Rapid SARS-CoV-2 Diagnosis Using Disposable Strips and a Metal-oxide-semiconductor Field-effect Transistor Platform, Journal of Vacuum Science & Technology B, 2022, doi.org/10.1116/6.0001615, 40, 023204. [^‡Senior author] [View] [News]
	Nguyen LT, Macaluso NC, and Jain PK* A Combinatorial Approach towards Adaptability of 22 Functional Cas12a Orthologs for Nucleic Acid Detection in Clinical Samples medRxiv, 2021, https://doi.org/10.1101/2021.07.21.21260653 [*Corresponding author] [Manuscript reviewed, Science Advances] [Download pdf] [View]
	Nguyen LT, Gurijala, J, Rananaware SR, Pizzano BLM, Brandon ST, and Jain PK* CRISPR-ENHANCE: An enhanced nucleic acid detection platform using Cas12a. *Methods,* 2022, 10.1016/j.ymeth.2021.02.001 [*Corresponding author] [View]
	Shen Y, Ostrov DA, Santosh Rananaware SR, Jain PK^‡, Nguyen CQ* Identification of risk and protective human leukocyte antigens in COVID-19 using genotyping and structural modeling medRxiv, 2021, https://doi.org/10.1101/2021.05.04.21256636 [^‡Senior author] [Download pdf] [View]
	Nguyen LT, Smith,BM, Jain PK* Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection Nature Communications, 2020, 11, 4906 [*Corresponding author] [Download pdf] [View] [BioRxiv version]
	Downing M and Jain PK, Mesoporous silica nanoparticles: synthesis, properties, and biomedical applications. Nanoparticles for Biomedical Applications,* 2019; 267-281, Book Chapter. [*Corresponding author] [View]
	Jain PK, Lo JH, Rananaware S, Downing M, Panda A, Tai M, Raghavan S, Fleming HE and Bhatia SN. Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes. *Nanoscale, 2019;* 11, 21317-21323 [*Co-corresponding author] [Download pdf] [View]
	Jain PK, and Friedman SH. A universal photo-cleavable clickable reagent for the reversible end labeling of any nucleic acid. ChemBioChem, 2018;* 19, 1264. [Download pdf] [View] [F1000Prime recommendation] [Very Important Paper][ChemistryViews][Cover]
	Jain PK, Ramanan V, Schepers AG, Dalvie N, Panda A, Fleming HE and Bhatia SN. Development of light-activated CRISPR using photocleavable protectors of guide RNAs. Angewandte Chemie International Edition, 2016;* 55, 12440. [Download pdf] [View] [News articles] [Altmetrics]
	Sarode BR, Jain PK and Friedman SH. Polymerizing Insulin with Photocleavable Linkers to Make Light-Sensitive Macropolymer Depot Materials. Macromolecular Bioscience, 2016;* 16 (8): 1138-1146. [Download pdf] [View] [Featured on the cover]
	Dudani JS, Jain PK, Kwong GA, Stevens KR and Bhatia SN. Photoactivated Spatiotemporally-Responsive Nanosensors of in Vivo Protease Activity. ACS Nano, 2015;* 9(12), 11708-11717. [Download pdf] [View]
	Kala A, Jain PK and Friedman SH. Patterning of cells through patterning of biology. Molecular BioSystems*, 2014;10, 1689-1692. [Download pdf] [View]
	Kala A, Jain PK, Karunakaran D, Shah S and Friedman SH. The synthesis of tetra-modified RNA for the multidimensional control of gene expression via light-activated RNA interference. Nature protocols,* 2014; 9(1), 11-20. [Download pdf] [View]
	Jain PK, Karunakaran D and Friedman SH. Construction of a Photoactivated Insulin Depot. Angewandte Chemie International Edition, 2013*; 52, 1404-1409. [Download pdf] [View] [Featured on the frontispiece] [News articles]
	Jain PK, Shah S and Friedman SH. Patterning of gene expression using new photolabile groups applied to light activated RNAi. Journal of the American Chemical Society, 2011;* 133(3), 440-446. [Download pdf] [View]
	Shah S, Jain PK, Kala A, Karunakaran D and Friedman SH. Light-activated RNA interference using double-stranded siRNA precursors modified using a remarkable regiospecificity of diazo-based photolabile groups. Nucleic Acids Research, 2009;* 37(13), 4508-4517. [Download pdf] [View] [Featured on the cover]

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