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Yiider Tseng Associate Professor Ph.D., 1999, The Johns Hopkins University Interactomics Systems biology approaches Molecular Biomechanics Email: ytseng@che.ufl.edu Phone: (352) 392-0862 223 CHE

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Brief Description of Current Research

Grounded in science and engineering fundamentals, research in my laboratory focuses on combining new engineering principals with advanced life science methods to develop a systematic, quantitative, and integrative way of understand fundamental biological phenomena at the molecular and cellular levels. My research has implications in tissue engineering, wound repairs, microorganism invasions and disease states such as cancer metastasis. My lab is focused on three main areas of research:

1. Developing high-throughput methods to establish the complete "interactome" of the recently discovered bacterial cytoskeleton. After identifying regulators of the cytoskeleton, we will be able to pursue new molecular strategies to prevent bacterial invasion processes.
    
2. Combining micromanipulation and systems biology approaches to elucidate the distribution and function of lipids in cellular processes. Total internal reflection (TIRF) microscopy combined with cellular engineering will help us understand the relation between spatial and temporal micro-heterogeneity of the cell membrane and the roles of lipids in regulating cellular activities.
    
3. Applying in vivo multiple-particle tracking microrheology to study cell-mechanical mechanisms where force plays an essential role. We will focus on the role that force plays in regulating drug delivery, viral infection and bacterial invasion.
    

Selected Publications

• Repeated Rapid Shear-Responsiveness of a Peptide Hydrogel", by S. Ramachandran, Y. Tseng and Y. B. Yu, Biomacromolecules, Vol. 6, Issue 3, pp. 1316-1321 (2005).
   
• The Assembly of MreB, a Prokaryotic Homolog of Actin" by O. Esue, M. Cordero, D. Wirtz and Y. Tseng, Journal of Biological Chemistry, Vol. 280, No. 4, pp. 2628-2635 (2005).
   
• Cdc42 Mediates Nucleus Movement and MTOC Polarization in Swiss 3T3 Fibroblasts under Mechanical Shear Stress", by J.S.H. Lee, M.I. Chang, Y. Tseng and D. Wirtz, Molecular Biology of the Cell, Vol. 16, Issue 2, pp. 871-880 (2005).
   
• Intracellular Mechanics of Migrating Fibroblasts", by T. P. Kole, Y. Tseng, I. Chiang, J. L. Katz and D. Wirtz, Molecular Biology of the Cell, Vol. 16, Issue 1, pp. 328-338 (2005).
   
• Dendritic Branching and Homogenization of Actin Networks Mediated by Arp2/3 Complex", by Y. Tseng and D. Wirtz, Physical Review Letters, Vol. 93, No. 258104 (2004).
   
• Nuclear Envelope Breakdown Requires Overcoming the Mechanical Integrity of the Nuclear Lamina", by P. Panorchan, B. W. Schafer, D. Wirtz and Y. Tseng, Journal of Biological Chemistry, Vol. 279, No. 42, pp. 43462-43467 (2004).
   
• Rho Kinase Regulates the Intracellular Micromechanical Response of Adherent Cells to Rho Activation", by T. P. Kole, Y. Tseng, L. Huang, J. L. Katz and D. Wirtz, Molecular Biology of the Cell, Vol. 15, Issue 7, pp. 3475-3484 (2004).
   
• Micro-Organization and Visco-Elasticity of the Interphase Nucleus Revealed by Particle Nanotracking", by Y. Tseng, J. S. H. Lee, T. P. Kole, I. Jiang and D. Wirtz, Journal of Cell Science, Vol. 117, Issue 10, pp. 2159-2167 (2004).